UBE2G1 antibody (AA 101-170) (AbBy Fluor® 594)

Catalog No. ABIN2807344

The Rabbit Polyclonal anti-UBE2G1 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect UBE2G1 in samples from Rat.

Quick Overview for UBE2G1 antibody (AA 101-170) (AbBy Fluor® 594) (ABIN2807344)

Target: UBE2G1 (Ube2g1) (Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2G1 antibody is conjugated to AbBy Fluor® 594

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-UBE2G1 Antibody (AbBy Fluor® 594)

Binding Specificity: AA 101-170

Cross-Reactivity: Rat

Predicted Reactivity: Mouse,Dog,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Ube2G1/UBC7

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UBE2G1

Target: UBE2G1 (Ube2g1) (Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1))

Alternative Name: Ube2G1

Background:

Synonyms: E217K, UB2G1_HUMAN, UBC 7, UBC7, UBC7 homolog yeast, UBE 2G, UBE2G, Ube2g1, Ubiquitin carrier protein G1, Ubiquitin conjugating enzyme E2 G1, Ubiquitin conjugating enzyme E2G 1 homologous to C. elegans UBC7, Ubiquitin conjugating enzyme E2G 1 UBC7 homolog C. elegans, Ubiquitin conjugating enzyme E2G 1 UBC7 homolog yeast, Ubiquitin conjugating enzyme E2G 1, Ubiquitin protein ligase G1, Ubiquitin-conjugating enzyme E2 G1, Ubiquitin-protein ligase G1.

Background: Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign, and improperly folded proteins by targeting them for proteosome degradation. In Saccharomyces cerevisiae, ubiquitin-like proteins include Rub1, Ula1, Uba3, Smt3, Ubc2, Ubc12 and Ubc9. Rub1 shares 53 % homology with ubiquitin and requires activation via the E2 proteins, including Ula1, Uba3 and Ubc12 in order to conjugate to substrates directed to different proteolytic systems. Ubc4 catalyzes ubiquitination of IkBa in a phosphorylation and SCFB-TRCP dependent manner. In this particular reaction, E1 first transfers ubiquitin to the E2 component Ubc4, and Ubc4 then associates with E3 ligase, which conjugates the poly-ubiquitin chain on a target protein. In this fashion, the chain tags the IkBa for degradation by a proteasome thus lifting the inhibitory effect of IkBa on NFkB and allowing NFkB to enter the nucleus.

Gene ID: 7326