ATP6V1F antibody

Catalog No. ABIN2854351

This Rabbit Polyclonal antibody specifically detects ATP6V1F in WB. It exhibits reactivity toward Human.

Quick Overview for ATP6V1F antibody (ABIN2854351)

Target: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1F antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6V1F Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to V-ATPase F (ATPase, H+ transporting, lysosomal 14 kDa, V1 subunit F)
V-ATPase F antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human V-ATPase F. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATP6V1F

Target: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Alternative Name: ATPase H+ transporting V1 subunit F

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.

Molecular Weight: 13 kDa

Gene ID: 9296

UniProt: Q16864

Pathways: Transition Metal Ion Homeostasis, Proton Transport