STX1A antibody (N-Term)

Catalog No. ABIN2854559

The Rabbit Polyclonal anti-STX1A antibody has been validated for WB, IF and ICC. It is suitable to detect STX1A in samples from Human.

Quick Overview for STX1A antibody (N-Term) (ABIN2854559)

Target: STX1A (Syntaxin 1A (Brain) (STX1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STX1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-STX1A Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Rat

Characteristics: Rabbit polyclonal antibody to syntaxin 1A (syntaxin 1A (brain))
Syntaxin 1a antibody [N1], N-term

Purification: Affinity purified by Protein A.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Syntaxin 1a. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 9.3 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for STX1A

Target: STX1A (Syntaxin 1A (Brain) (STX1A))

Alternative Name: syntaxin 1A

Background: Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995 [PubMed 7622072]).[supplied by OMIM]

Cellular Localization: Cell membrane, Single-pass type IV membrane protein , Isoform 2: Secreted

Molecular Weight: 33 kDa

Gene ID: 6804

UniProt: Q16623

Pathways: Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport