KLC1 antibody

Catalog No. ABIN2854634

The Rabbit Polyclonal anti-KLC1 antibody has been validated for WB. It is suitable to detect KLC1 in samples from Human. There are 2+ publications available.

Quick Overview for KLC1 antibody (ABIN2854634)

Target: KLC1 (Kinesin Light Chain 1 (KLC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KLC1 Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to KLC1 (kinesin light chain 1)
KLC1 antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human KLC1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.42 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-KLC1 antibody (ABIN2854634)

Holt, Fuller, Lam, Sewry, Shirran, Zhang, Shanahan, Morris: "Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle." in: Scientific reports, Vol. 9, Issue 1, pp. 14202, (2019) (PubMed).

Carpentier, Gao, Ewles, Morgan, Smith: "Vaccinia virus protein complex F12/E2 interacts with kinesin light chain isoform 2 to engage the kinesin-1 motor complex." in: PLoS pathogens, Vol. 11, Issue 3, pp. e1004723, (2015) (PubMed).

Target Details for KLC1

Target: KLC1 (Kinesin Light Chain 1 (KLC1))

Alternative Name: kinesin light chain 1

Molecular Weight: 65 kDa

Gene ID: 3831

UniProt: Q07866

Pathways: Ribonucleoprotein Complex Subunit Organization