HSP90AB1 antibody (Internal Region)

Catalog No. ABIN2855037

This anti-HSP90AB1 antibody is a Rabbit Polyclonal antibody detecting HSP90AB1 in WB, IF, ICC and IHC (p). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for HSP90AB1 antibody (Internal Region) (ABIN2855037)

Target: HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP90AB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSP90AB1 Antibody

Binding Specificity: Internal Region

Cross-Reactivity: Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to HSP90 beta (heat shock protein 90 kDa alpha (cytosolic), class B member 1)
HSP90 beta antibody [N2C2], Internal

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HSP90 beta. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HeLaS3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.43 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HSP90AB1 antibody (ABIN2855037)

Galmozzi, Kok, Kim, Montenegro-Burke, Lee, Spreafico, Mosure, Albert, Cintron-Colon, Godio, Webb, Conti, Solt, Kojetin, Parker, Peluso, Pru, Siuzdak, Cravatt, Saez: "PGRMC2 is an intracellular haem chaperone critical for adipocyte function." in: Nature, Vol. 576, Issue 7785, pp. 138-142, (2020) (PubMed).

Target Details for HSP90AB1

Target: HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))

Alternative Name: HSP90 beta

Background: HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1, MIM 191175) and mitochondria (TRAP1, MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Cellular Localization: Cytoplasm , Melanosome

Molecular Weight: 83 kDa

Gene ID: 3326

Pathways: Regulation of Cell Size