PRKAG1 antibody (Center)

Catalog No. ABIN2855115

This anti-PRKAG1 antibody is a Rabbit Polyclonal antibody detecting PRKAG1 in WB and IP. Suitable for Human and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for PRKAG1 antibody (Center) (ABIN2855115)

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-PRKAG1 Antibody

Binding Specificity: Center

Cross-Reactivity: Pig (Porcine), Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Pig (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to AMPK gamma 1 (5'-AMP-activated protein kinase subunit gamma-1)
AMPK gamma 1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human AMPK gamma 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Immunoprecipitation Assay-dependent dilution Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunoprecipitationAssay-dependent dilution Western blot1:500-1:3000*

Comment:

Positive Control: mouse liver

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.33 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PRKAG1 antibody (ABIN2855115)

Lin, Kiihl, Suhail, Liu, Chou, Kuang, Lu, Khor, Lin, Bader, Irizarry, Boeke: "Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK." in: Nature, Vol. 482, Issue 7384, pp. 251-5, (2012) (PubMed).

Target Details for PRKAG1

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Alternative Name: AMPK gamma 1

Background: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Molecular Weight: 38 kDa

Gene ID: 5571

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process, Warburg Effect