alpha Actinin 4 antibody (Actinin, alpha 4) (C-Term)

Details for Product anti-ACTN4 Antibody No. ABIN2855119
Antigen
Epitope
C-Term
17
11
9
5
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
115
46
43
8
5
5
4
4
3
3
2
2
1
1
1
Host
Rabbit
91
24
Clonality
Polyclonal
Conjugate
This alpha Actinin 4 antibody is un-conjugated
5
3
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
97
50
50
19
17
17
11
7
4
3
2
2
1
1
1
1
1
Options
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human alpha Actinin 4. The exact sequence is proprietary.
Isotype IgG
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to alpha Actinin 4 (actinin, alpha 4)
alpha Actinin 4 antibody [C2C3], C-term
Purification Purified by antigen-affinity chromatography.
Antigen
Alternative Name alpha Actinin 4 (ACTN4 Antibody Abstract)
Background Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Cellular Localization: Nucleus , Cytoplasm
Molecular Weight 105 kDa
Gene ID 81
Pathways Proton Transport
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , A375 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , mouse brain , PC-12

Restrictions For Research Use only
Format Liquid
Concentration 0.32 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) WB Image alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by Western blo...
Image no. 2 for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) IHC-P Image alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein o...
Image no. 3 for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) WB Image alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by Western blo...
Image no. 4 for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE antibody dilu...
Image no. 5 for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) IHC-P Image Immunohistochemical analysis of paraffin-embedded A549 xenograft, using a...
Image no. 6 for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) WB Image alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by Western blo...
Image no. 7 for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...