alpha Actinin 4 antibody (C-Term)

Catalog No. ABIN2775289

Cited in 1 publication. The Rabbit Polyclonal anti-alpha Actinin 4 antibody (ABIN2775289) specifically detects alpha Actinin 4 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig and Zebrafish (Danio rerio) samples.

Quick Overview for alpha Actinin 4 antibody (C-Term) (ABIN2775289)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Actinin 4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-alpha Actinin 4 Antibody

Binding Specificity: C-Term

Purpose: ACTN4 Antibody - C-terminal region

Sequence: DVENDRQGEA EFNRIMSLVD PNHSGLVTFQ AFIDFMSRET TDTDTADQVI

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Human: 100%, Mouse: 93%, Rat: 93%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ACTN4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ACTN4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8093885

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-alpha Actinin 4 antibody (ABIN2775289)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: ACTN4

Background:

Background Information: Alpha actinins belong to the spectrin superfamily which is a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. ACTN4 is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in its gene have been associated with focal and segmental glomerulosclerosis.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Gene Name: Actinin, alpha 4

Alternative Symbols: FSGS, FSGS1, ACTININ-4

Protein Name: Alpha-actinin-4

Molecular Weight: 105kDa

Gene ID: 81

NCBI Accession: NP_004915

UniProt: O43707

Pathways: Proton Transport