CD81 antibody

Catalog No. ABIN2855163

This anti-CD81 antibody is a Rabbit Polyclonal antibody detecting CD81 in FACS, WB, IF, IHC (p), ICC and BP. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CD81 antibody (ABIN2855163)

Target: CD81

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Blocking Peptide (BP)

Grade: KO Validated

Product Details anti-CD81 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to CD81 (CD81 Molecule)
CD81 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CD81. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.01 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CD81 antibody (ABIN2855163)

Chen, Lin, Tsai, He, Luo, Chiu, Herschman, Li: "Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain." in: Stem cells translational medicine, Vol. 9, Issue 4, pp. 499-517, (2020) (PubMed).

Kuo, Hsieh, Wang, Chang, Hung, Kuo, Liu, Li, Liu: "Simvastatin Attenuates Cardiac Fibrosis via Regulation of Cardiomyocyte-Derived Exosome Secretion." in: Journal of clinical medicine, Vol. 8, Issue 6, (2019) (PubMed).

Target Details for CD81

Target: CD81

Alternative Name: CD81 molecule

Background: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies.

Cellular Localization: Membrane

Molecular Weight: 26 kDa

Gene ID: 975

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C