ACTC1 antibody

Catalog No. ABIN2855212

This Rabbit Polyclonal antibody specifically detects ACTC1 in WB, ICC, IHC (p) and IF. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for ACTC1 antibody (ABIN2855212)

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-ACTC1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to alpha Cardiac Muscle Actin (actin, alpha, cardiac muscle 1)
alpha Cardiac Muscle Actin antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Cardiac Muscle Actin. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: PC-12 , Rat2 , Jurkat , Raji , K562 , THP-1 , NIH-3T3 , JC , BCL-1 , Rat muscle

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.56 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ACTC1 antibody (ABIN2855212)

Henry, Szabó, Sutus, Pirity: "RYBP is important for cardiac progenitor cell development and sarcomere formation." in: PLoS ONE, Vol. 15, Issue 7, pp. e0235922, (2020) (PubMed).

Oldershaw, Owens, Sutherland, Linney, Liddle, Magana, Lash, Gill, Richardson, Meeson: "Human Cardiac-Mesenchymal Stem Cell-Like Cells, a Novel Cell Population with Therapeutic Potential." in: Stem cells and development, Vol. 28, Issue 9, pp. 593-607, (2020) (PubMed).

Miksiunas, Rucinskas, Janusauskas, Labeit, Bironaite: "Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Improves Energetic Status and Cardiomyogenic Differentiation of Human Dilated Myocardium-Derived Primary Mesenchymal Cells." in: International journal of molecular sciences, Vol. 21, Issue 14, (2020) (PubMed).

Robertson, Wen, Yin, Chen, Smith, Paine: "Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells." in: Frontiers in physiology, Vol. 8, pp. 336, (2017) (PubMed).

Target Details for ACTC1

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Alternative Name: actin alpha cardiac muscle 1

Background: Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

Cellular Localization: Cytoplasm , cytoskeleton

Molecular Weight: 42 kDa

Gene ID: 70

UniProt: P68032

Pathways: Myometrial Relaxation and Contraction