CARD8 antibody (C-Term)

Catalog No. ABIN2855381

The Rabbit Polyclonal anti-CARD8 antibody has been validated for WB, IF and ICC. It is suitable to detect CARD8 in samples from Human.

Quick Overview for CARD8 antibody (C-Term) (ABIN2855381)

Target: CARD8 (Caspase Recruitment Domain Family, Member 8 (CARD8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CARD8 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to CARD8 (caspase recruitment domain family, member 8)
CARD8 antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human CARD8. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 40 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for CARD8

Target: CARD8 (Caspase Recruitment Domain Family, Member 8 (CARD8))

Alternative Name: caspase recruitment domain family member 8

Background: The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene, but their biological validity has not been determined.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 49 kDa

Gene ID: 22900

UniProt: Q9Y2G2

Pathways: Positive Regulation of Endopeptidase Activity