Keratin 2 antibody

Catalog No. ABIN2855477

The Rabbit Polyclonal anti-Keratin 2 antibody has been validated for WB, ICC, IF and IHC (p). It is suitable to detect Keratin 2 in samples from Human.

Quick Overview for Keratin 2 antibody (ABIN2855477)

Target: Keratin 2 (KRT2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Keratin 2 Antibody

Cross-Reactivity: Dog, Human

Characteristics: Rabbit Polyclonal antibody to Cytokeratin 2 (keratin 2)
Cytokeratin 2 antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytokeratin 2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.67 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Keratin 2

Target: Keratin 2 (KRT2)

Alternative Name: keratin 2

Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Molecular Weight: 65 kDa

Gene ID: 3849

UniProt: P35908