DDX6 antibody (Center)

Catalog No. ABIN2855483

The Rabbit Polyclonal anti-DDX6 antibody has been validated for WB, IHC (p), IF and ICC. It is suitable to detect DDX6 in samples from Human and Mouse.

Quick Overview for DDX6 antibody (Center) (ABIN2855483)

Target: DDX6 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DDX6 Antibody

Binding Specificity: Center

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to DDX6 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 6)
DDX6 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DDX6. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.708 mg/mL

Buffer: 1XPBS, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DDX6

Target: DDX6 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6))

Alternative Name: DDX6

Background: This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.

Cellular Localization: Cytoplasm , P-body

Molecular Weight: 54 kDa

Gene ID: 1656

Pathways: Ribonucleoprotein Complex Subunit Organization