LDH antibody

Catalog No. ABIN285556

This Goat Polyclonal antibody specifically detects in IP, ELISA and WB. It exhibits reactivity toward Rabbit and has been mentioned in 6+ publications.

Quick Overview for LDH antibody (ABIN285556)

Target: LDH

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), ELISA, Western Blotting (WB)

Product Details

Purification: LDH antibody was purified by delipidation and defibrination.

Immunogen: LDH antibody was raised in goat using full length lactate dehydrogenase protein isolated from rabbit muscle as the immunogen.

Application Details

Application Notes: ELISA: 1:4,000-1:20,000, IP: 1:100, WB: 1:500-1:2,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: In lyophilized form PBS buffer with 0.01 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: -20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage

References

Uddin, Zou, Sharma, Gatla, Vancurova: "Proteasome inhibition induces IKK-dependent interleukin-8 expression in triple negative breast cancer cells: Opportunity for combination therapy." in: PLoS ONE, Vol. 13, Issue 8, pp. e0201858, (2019) (PubMed).

Sanacora, Urdinez, Chang, Vancurova: "Anticancer drug bortezomib increases interleukin-8 expression in human monocytes." in: Biochemical and biophysical research communications, Vol. 460, Issue 2, pp. 375-9, (2015) (PubMed).

Chang, Poltoratsky, Vancurova: "Bortezomib inhibits expression of TGF-β1, IL-10, and CXCR4, resulting in decreased survival and migration of cutaneous T cell lymphoma cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 6, pp. 2942-53, (2015) (PubMed).

Chang, Vancurova: "Bcl3 regulates pro-survival and pro-inflammatory gene expression in cutaneous T-cell lymphoma." in: Biochimica et biophysica acta, Vol. 1843, Issue 11, pp. 2620-30, (2014) (PubMed).

Manna, Singha, Phyo, Gatla, Chang, Sanacora, Ramaswami, Vancurova: "Proteasome inhibition by bortezomib increases IL-8 expression in androgen-independent prostate cancer cells: the role of IKKα." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 5, pp. 2837-46, (2013) (PubMed).

Juvekar, Manna, Ramaswami, Chang, Vu, Ghosh, Celiker, Vancurova: "Bortezomib induces nuclear translocation of IκBα resulting in gene-specific suppression of NF-κB--dependent transcription and induction of apoptosis in CTCL." in: Molecular cancer research : MCR, Vol. 9, Issue 2, pp. 183-94, (2011) (PubMed).

Target Details

Target: LDH