RANBP1 antibody

Catalog No. ABIN2855572

This Rabbit Polyclonal antibody specifically detects RANBP1 in WB, IF and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for RANBP1 antibody (ABIN2855572)

Target: RANBP1 (RAN Binding Protein 1 (RANBP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RANBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-RANBP1 Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to Ran BP1 (RAN binding protein 1)
Ran BP1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RanBP1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , HeLaS3 , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-RANBP1 antibody (ABIN2855572)

Chao, Lai, Lu, Lin, Mai, Huang: "NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3." in: Nucleic acids research, Vol. 40, Issue 17, pp. 8484-98, (2012) (PubMed).

Target Details for RANBP1

Target: RANBP1 (RAN Binding Protein 1 (RANBP1))

Alternative Name: RAN binding protein 1

Background: Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92 % similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Molecular Weight: 23 kDa

Gene ID: 5902

UniProt: P43487