BIN1 antibody

Catalog No. ABIN2855595

This anti-BIN1 antibody is a Rabbit Polyclonal antibody detecting BIN1 in WB, IHC, IF, ICC and IHC (p). Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for BIN1 antibody (ABIN2855595)

Target: BIN1 (Bridging Integrator 1 (BIN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BIN1 Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to BIN1 (bridging integrator 1)
BIN1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human BIN1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Comparison

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.35 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-BIN1 antibody (ABIN2855595)

Chan Wah Hak, Khan, Di Meglio, Law, Lucken-Ardjomande Häsler, Quintaneiro, Ferreira, Krause, McMahon, Boucrot: "FBP17 and CIP4 recruit SHIP2 and lamellipodin to prime the plasma membrane for fast endophilin-mediated endocytosis." in: Nature cell biology, Vol. 20, Issue 9, pp. 1023-1031, (2019) (PubMed).

Target Details for BIN1

Target: BIN1 (Bridging Integrator 1 (BIN1))

Alternative Name: bridging integrator 1

Background: This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

Cellular Localization: Isoform BIN1: Nucleus , Isoform IIA: Cytoplasm

Molecular Weight: 65 kDa

Gene ID: 274

UniProt: O00499