NSMAF antibody

Catalog No. ABIN2855689

This Rabbit Polyclonal antibody specifically detects NSMAF in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for NSMAF antibody (ABIN2855689)

Target: NSMAF (Neutral Sphingomyelinase (N-SMase) Activation Associated Factor (NSMAF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSMAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NSMAF Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to FAN (neutral sphingomyelinase (N-SMase) activation associated factor)
FAN antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human FAN. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NSMAF transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.93 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NSMAF

Target: NSMAF (Neutral Sphingomyelinase (N-SMase) Activation Associated Factor (NSMAF))

Alternative Name: neutral sphingomyelinase activation associated factor

Background: This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.

Molecular Weight: 104 kDa

Gene ID: 8439

UniProt: Q92636