ETS2 antibody (Center)

Catalog No. ABIN2855845

This Rabbit Polyclonal antibody specifically detects ETS2 in WB, IF, IHC (p), IP, ChIP and ICC. It exhibits reactivity toward Human and Rat and has been mentioned in 3+ publications.

Quick Overview for ETS2 antibody (Center) (ABIN2855845)

Target: ETS2 (V-Ets Erythroblastosis Virus E26 Oncogene Homolog 2 (ETS2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)

Product Details anti-ETS2 Antibody

Binding Specificity: Center

Characteristics: Rabbit Polyclonal antibody to ETS2 (v-ets erythroblastosis virus E26 oncogene homolog 2 (avian))
ETS2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ETS2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ETS2 antibody (ABIN2855845)

Gluck, Glathar, Tsompana, Nowak, Garrett-Sinha, Buck, Sinha: "Molecular dissection of the oncogenic role of ETS1 in the mesenchymal subtypes of head and neck squamous cell carcinoma." in: PLoS genetics, Vol. 15, Issue 7, pp. e1008250, (2019) (PubMed).

Yuan, Jiang, Mayakonda, Huang, Ding, Lin, Yu, Lu, Loh, Chow, Savage, Tyner, Lin, Koeffler: "Super-Enhancers Promote Transcriptional Dysregulation in Nasopharyngeal Carcinoma." in: Cancer research, Vol. 77, Issue 23, pp. 6614-6626, (2018) (PubMed).

Helbig, Wockner, Bouendeu, Hille-Betz, McCue, French, Edwards, Pickett, Reddel, Chenevix-Trench, Dörk, Beesley: "Functional dissection of breast cancer risk-associated TERT promoter variants." in: Oncotarget, Vol. 8, Issue 40, pp. 67203-67217, (2017) (PubMed).

Target Details for ETS2

Target: ETS2 (V-Ets Erythroblastosis Virus E26 Oncogene Homolog 2 (ETS2))

Alternative Name: ETS2

Background: ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]

Cellular Localization: Nucleus

Molecular Weight: 53 kDa

Gene ID: 2114

Pathways: EGFR Signaling Pathway, Myometrial Relaxation and Contraction