PD-L1 antibody (C-Term)

Catalog No. ABIN2855940

This anti-PD-L1 antibody is a Rabbit Polyclonal antibody detecting PD-L1 in WB, FACS, IF, ICC, IHC (p) and IHC (fro). Suitable for Human. This Primary Antibody has been cited in 7+ publications.

Quick Overview for PD-L1 antibody (C-Term) (ABIN2855940)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade: KO Validated

Product Details anti-PD-L1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to PD-L1 (CD274 Molecule)
PD-L1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PD-L1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MDA-MB-231 (24μg/ml Tunicamycin treatment for 16 hr) , MDA-MB-231 , A431(100 ng/mL IFN-gamma treatment for 48 hr)

Validation: Comparison, KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.29 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PD-L1 antibody (ABIN2855940)

Kim, Kim, Kim, Kim, Kim: "Differential Expression and Clinicopathological Significance of HER2, Indoleamine 2,3-Dioxygenase and PD-L1 in Urothelial Carcinoma of the Bladder." in: Journal of clinical medicine, Vol. 9, Issue 5, (2020) (PubMed).

Qin, Wang, Ye, Li, Chen, Wang, Qin, Zhang, Li, Long, Hu, Shi, Li, Zhang, Zhai, Tang, Kang, Lan, Xie, Lu, Deng: "NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer." in: Nature communications, Vol. 11, Issue 1, pp. 1669, (2020) (PubMed).

Gondhowiardjo, Handoko, Adham, Rachmadi, Kodrat, Tobing, Haryoga, Dwiyono, Kristian, Mayang Permata: "Tumor microenvironment predicts local tumor extensiveness in PD-L1 positive nasopharyngeal cancer." in: PLoS ONE, Vol. 15, Issue 3, pp. e0230449, (2020) (PubMed).

Pan, Wu, Kan, Li, Chang, Wu, Li, Ou-Yang, Hou, Yip, Luo: "Impact of FAK Expression on the Cytotoxic Effects of CIK Therapy in Triple-Negative Breast Cancer." in: Cancers, Vol. 12, Issue 1, (2019) (PubMed).

Cha, Yang, Xia, Wei, Chan, Lim, Li, Kim, Chang, Lee, Hsu, Wang, Kuo, Chang, Hadad, Purdie, McCoy, Cai, Tu, Litton, Mittendorf, Moulder, Symmans, Thompson, Piwnica-Worms, Chen, Khoo, Hung: "Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1." in: Molecular cell, Vol. 71, Issue 4, pp. 606-620.e7, (2019) (PubMed).

Buisseret, Garaud, de Wind, Van den Eynden, Boisson, Solinas, Gu-Trantien, Naveaux, Lodewyckx, Duvillier, Craciun, Veys, Larsimont, Piccart-Gebhart, Stagg, Sotiriou, Willard-Gallo: "Tumor-infiltrating lymphocyte composition, organization and PD-1/ PD-L1 expression are linked in breast cancer." in: Oncoimmunology, Vol. 6, Issue 1, pp. e1257452, (2017) (PubMed).

Lin, Sung, Hsieh, Tsai, Lai, Yang, Shen, Chen, Lee, Yeh, Chen: "High PD-L1 Expression Correlates with Metastasis and Poor Prognosis in Oral Squamous Cell Carcinoma." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142656, (2016) (PubMed).

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274 molecule

Background: Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Cellular Localization: Isoform 1: Cell membrane, Single-pass type I membrane protein , Isoform 2: Endomembrane system, Single-pass type I membrane protein

Molecular Weight: 33 kDa

Gene ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints