Flotillin 1 antibody (C-Term)

Catalog No. ABIN2855944

This Rabbit Polyclonal antibody specifically detects Flotillin 1 in WB and IP. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for Flotillin 1 antibody (C-Term) (ABIN2855944)

Target: Flotillin 1 (FLOT1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Flotillin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-Flotillin 1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Hamster, Human

Characteristics: Rabbit polyclonal antibody to Flotillin 1 (flotillin 1)
Flotillin 1 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.79 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Flotillin 1 antibody (ABIN2855944)

Pérez-Rodriguez, de Jesús Ramírez-Lira, Wulff, Voldbor, Ramírez, Trujillo-Roldán, Valdez-Cruz: "Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis." in: PLoS ONE, Vol. 15, Issue 8, pp. e0237930, (2020) (PubMed).

Rivera-Serrano, González-López, Das, Lemon: "Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses." in: eLife, Vol. 8, (2019) (PubMed).

Target Details for Flotillin 1

Target: Flotillin 1 (FLOT1)

Alternative Name: flotillin 1

Background: Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

Cellular Localization: Cell membrane, Peripheral membrane protein , Membrane , caveola, Peripheral membrane protein , Melanosome

Molecular Weight: 47 kDa

Gene ID: 10211

UniProt: O75955