LOXL2 antibody

Catalog No. ABIN2856034

This Rabbit Polyclonal antibody specifically detects LOXL2 in WB, IHC, ELISA, ICC, IHC (p) and IF. It exhibits reactivity toward Human and has been mentioned in 5+ publications.

Quick Overview for LOXL2 antibody (ABIN2856034)

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LOXL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-LOXL2 Antibody

Specificity: Mouse reactivity has been validated by IHC-P only, not yet been tested by other applications.

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to LOXL2 (lysyl oxidase-like 2)
LOXL2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG , SK-N-SH , SK-N-AS , 293T transfected with LOXL2

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.86 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-LOXL2 antibody (ABIN2856034)

Cao, Lin, Zhi, Lazare, Meng, Wu, Gao, Wei, Wu: "LOXL2 Expression Status Is Correlated With Molecular Characterizations of Cervical Carcinoma and Associated With Poor Cancer Survival via Epithelial-Mesenchymal Transition (EMT) Phenotype." in: Frontiers in oncology, Vol. 10, pp. 284, (2020) (PubMed).

Mahjour, Dambal, Shrestha, Singh, Noonan, Kantarci, Trackman: "Mechanism for oral tumor cell lysyl oxidase like-2 in cancer development: synergy with PDGF-AB." in: Oncogenesis, Vol. 8, Issue 5, pp. 34, (2019) (PubMed).

Wang, Zhao, Zhu, Liu, Liang, Liu, Zhang, Dong, Sun: "HIF-1α promoted vasculogenic mimicry formation in hepatocellular carcinoma through LOXL2 up-regulation in hypoxic tumor microenvironment." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 60, (2018) (PubMed).

Aumiller, Strobel, Romeike, Schuler, Stierstorfer, Kreuz: "Comparative analysis of lysyl oxidase (like) family members in pulmonary fibrosis." in: Scientific reports, Vol. 7, Issue 1, pp. 149, (2018) (PubMed).

Bais, Kukuruzinska, Trackman: "Orthotopic non-metastatic and metastatic oral cancer mouse models." in: Oral oncology, Vol. 51, Issue 5, pp. 476-82, (2015) (PubMed).

Target Details for LOXL2

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Alternative Name: lysyl oxidase like 2

Background: This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.

Cellular Localization: Secreted , extracellular space

Molecular Weight: 87 kDa

Gene ID: 4017

UniProt: Q9Y4K0

Pathways: Chromatin Binding