BCL2L1 antibody

Catalog No. ABIN2856151

This Rabbit Polyclonal antibody specifically detects BCL2L1 in WB, IF, IP, IHC (p) and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for BCL2L1 antibody (ABIN2856151)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL2L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-BCL2L1 Antibody

Cross-Reactivity: Dog, Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Bcl-X (BCL2-like 1)
Bcl-X antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Bcl-X. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , PC-12

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-BCL2L1 antibody (ABIN2856151)

Wier, Fu, Hodgson, Sun, Wan: "Caspase-3 cleaved p65 fragment dampens NF-κB-mediated anti-apoptotic transcription by interfering with the p65/RPS3 interaction." in: FEBS letters, Vol. 589, Issue 23, pp. 3581-7, (2016) (PubMed).

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: BCL2 like 1

Background: The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

Cellular Localization: Mitochondrion membrane, Single-pass membrane protein , Nucleus membrane, Single-pass membrane protein, Cytoplasmic side , Mitochondrion membrane , Nucleus membrane

Molecular Weight: 26 kDa

Gene ID: 598

UniProt: Q07817

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling