RNGTT antibody

Catalog No. ABIN2856163

This Rabbit Polyclonal antibody specifically detects RNGTT in WB. It exhibits reactivity toward Human.

Quick Overview for RNGTT antibody (ABIN2856163)

Target: RNGTT (RNA Guanylyltransferase and 5'-Phosphatase (RNGTT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNGTT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNGTT Antibody

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to HCE (RNA guanylyltransferase and 5'-phosphatase)
HCE antibody [N1N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HCE. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa , HepG2 , Molt-4

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.54 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RNGTT

Target: RNGTT (RNA Guanylyltransferase and 5'-Phosphatase (RNGTT))

Alternative Name: RNA guanylyltransferase and 5'-phosphatase

Background: Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.

Cellular Localization: Nucleus

Molecular Weight: 69 kDa

Gene ID: 8732

UniProt: O60942