NDUFS3 antibody (Center)

Catalog No. ABIN2856200

Product Details anti-NDUFS3 Antibody

Target: NDUFS3 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 3, 30kDa (NADH-Coenzyme Q Reductase) (NDUFS3))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDUFS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Characteristics: Rabbit Polyclonal antibody to NDUFS3 (NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30 kDa (NADH-coenzyme Q reductase))
NDUFS3 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NDUFS3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: Jurkat , Raji , NCI-H929

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-NDUFS3 antibody (ABIN2856200)

Qiao, Jin, Pang, Moskophidis, Mivechi: "The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis." in: The Journal of cell biology, Vol. 216, Issue 3, pp. 723-741, (2017) (PubMed).

Target Details for NDUFS3

Target: NDUFS3 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 3, 30kDa (NADH-Coenzyme Q Reductase) (NDUFS3))

Alternative Name: NDUFS3 (NDUFS3 Products)

Background: This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

Cellular Localization: Mitochondrion inner membrane

Molecular Weight: 30 kDa

Gene ID: 4722

Pathways: Negative Regulation of intrinsic apoptotic Signaling