HSPA1B antibody (Center)

Catalog No. ABIN2856263

The Rabbit Polyclonal anti-HSPA1B antibody has been validated for WB, ICC, IF and IHC (p). It is suitable to detect HSPA1B in samples from Human and Rat.

Quick Overview for HSPA1B antibody (Center) (ABIN2856263)

Target: HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPA1B antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSPA1B Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Mouse (97 %)

Characteristics: Rabbit Polyclonal antibody to HSP70 1B (heat shock 70 kDa protein 1B)
HSP70 1B antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HSP70 1B. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:500-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:500-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HSPA1B

Target: HSPA1B (Heat Shock 70kDa Protein 1B (HSPA1B))

Alternative Name: HSPA1B

Background: This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Molecular Weight: 70 kDa

Gene ID: 3304