POLR3A antibody (N-Term)

Catalog No. ABIN2856286

This Rabbit Polyclonal antibody specifically detects POLR3A in WB, ICC, IF and IP. It exhibits reactivity toward Human.

Quick Overview for POLR3A antibody (N-Term) (ABIN2856286)

Target: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3A antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-POLR3A Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine), Chicken, Xenopus laevis, Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Mouse (97 %), Chicken (97 %), Xenopus laevis (94 %), Bovine (98 %), Xenopus tropicalis (94 %)

Characteristics: Rabbit Polyclonal antibody to POLR3A (polymerase (RNA) III (DNA directed) polypeptide A, 155 kDa)
POLR3A antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human POLR3A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: HCT116 , SK-N-SH , IMR32 , SK-N-AS

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.49 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for POLR3A

Target: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Alternative Name: POLR3A

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Cellular Localization: Nucleus

Molecular Weight: 156 kDa

Gene ID: 11128