IPO7 antibody (C-Term)

Catalog No. ABIN2856366

This anti-IPO7 antibody is a Rabbit Polyclonal antibody detecting IPO7 in WB, IHC (p), IF and ICC. Suitable for Human.

Quick Overview for IPO7 antibody (C-Term) (ABIN2856366)

Target: IPO7 (Importin 7 (IPO7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IPO7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-IPO7 Antibody

Binding Specificity: C-Term

Specificity: Knockdown/Knockout validation was supported by references (PMID:25999477)

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to Importin 7 (importin 7)
Importin 7 antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Importin 7. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for IPO7

Target: IPO7 (Importin 7 (IPO7))

Alternative Name: importin 7

Background: The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 120 kDa

Gene ID: 10527

UniProt: O95373

Pathways: Protein targeting to Nucleus