BPGM antibody

Catalog No. ABIN2856491

The Rabbit Polyclonal anti-BPGM antibody has been validated for WB, ICC and IF. It is suitable to detect BPGM in samples from Human.

Quick Overview for BPGM antibody (ABIN2856491)

Target: BPGM (2,3-bisphosphoglycerate Mutase (BPGM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BPGM antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-BPGM Antibody

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to BPGM (2,3-bisphosphoglycerate mutase)
BPGM antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human BPGM. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse spleen , 293T , A431 , H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.84 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BPGM

Target: BPGM (2,3-bisphosphoglycerate Mutase (BPGM))

Alternative Name: bisphosphoglycerate mutase

Background: 2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Molecular Weight: 30 kDa

Gene ID: 669

UniProt: P07738