CS antibody

Catalog No. ABIN2856769

This Rabbit Polyclonal antibody specifically detects CS in WB, IP, ICC, IF, IHC (p) and IHC (wm). It exhibits reactivity toward Human and has been mentioned in 5+ publications.

Quick Overview for CS antibody (ABIN2856769)

Target: CS (Citrate Synthase (CS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CS antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm))

Grade: KO Validated

Product Details anti-CS Antibody

Cross-Reactivity: Chicken, Frog, Human, Monkey, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to Citrate synthetase (citrate synthase)
Citrate synthetase antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse brain , rat brain , Rat muscle , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.65 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CS antibody (ABIN2856769)

Ding, Rose, Sun, Wu, Chen, Lin, Yang, Chen, Lee, Xu, Tian, Akinwuntan, Zhao, Guan, Zhou, Chi: "MESH1 is a cytosolic NADPH phosphatase that regulates ferroptosis." in: Nature metabolism, Vol. 2, Issue 3, pp. 270-277, (2020) (PubMed).

Leandro, Violante, Argmann, Hagen, Dodatko, Bender, Zhang, Williams, Bachmann, Auwerx, Yu, Houten: "Mild inborn errors of metabolism in commonly used inbred mouse strains." in: Molecular genetics and metabolism, Vol. 126, Issue 4, pp. 388-396, (2019) (PubMed).

Kim, Men, Liang, Receno, Brutsaert, Korol, Heffernan, DeRuisseau: "Effects of long-term exposures to low iron and branched-chain amino acid containing diets on aging skeletal muscle of Fisher 344 × Brown Norway rats." in: Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme, Vol. 43, Issue 2, pp. 165-173, (2018) (PubMed).

Nam, Akie, Sanosaka, Craige, Kant, Keaney, Cooper: "Mitochondrial retrograde signaling connects respiratory capacity to thermogenic gene expression." in: Scientific reports, Vol. 7, Issue 1, pp. 2013, (2018) (PubMed).

Chang, Ulrich, Suliman, Piantadosi: "Redox regulation of mitophagy in the lung during murine Staphylococcus aureus sepsis." in: Free radical biology & medicine, Vol. 78, pp. 179-89, (2015) (PubMed).

Target Details for CS

Target: CS (Citrate Synthase (CS))

Alternative Name: citrate synthase

Background: The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 52 kDa

Gene ID: 1431

UniProt: O75390