ATP6V1H antibody (Center)

Catalog No. ABIN2856776

The Rabbit Polyclonal anti-ATP6V1H antibody has been validated for WB, IHC (p), IF and ICC. It is suitable to detect ATP6V1H in samples from Human and Mouse.

Quick Overview for ATP6V1H antibody (Center) (ABIN2856776)

Target: ATP6V1H (ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ATP6V1H Antibody

Binding Specificity: Center

Cross-Reactivity: Chicken, Pig (Porcine), Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Chicken (96 %), Pig (99 %), Xenopus laevis (94 %), Zebrafish (91 %), Bovine (98 %), Xenopus tropicalis (94 %)

Characteristics: Rabbit Polyclonal antibody to ATP6V1H (ATPase, H+ transporting, lysosomal 50/57 kDa, V1 subunit H)
ATP6V1H antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IFAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLa , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.56 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATP6V1H

Target: ATP6V1H (ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H))

Alternative Name: ATP6V1H

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit.

Molecular Weight: 56 kDa

Gene ID: 51606

Pathways: Transition Metal Ion Homeostasis, Proton Transport