WNT10A antibody (Center)

Catalog No. ABIN2856795

This anti-WNT10A antibody is a Rabbit Polyclonal antibody detecting WNT10A in WB, ICC and IF. Suitable for Human.

Quick Overview for WNT10A antibody (Center) (ABIN2856795)

Target: WNT10A (Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT10A antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-WNT10A Antibody

Binding Specificity: Center

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine)

Cross-Reactivity (Details): Mouse (96 %), Rat (97 %), Bovine (96 %)

Characteristics: Rabbit polyclonal antibody to WNT10A (wingless-type MMTV integration site family, member 10A)
WNT10A antibody [N1C1-2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human WNT10A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for WNT10A

Target: WNT10A (Wingless-Type MMTV Integration Site Family, Member 10A (WNT10A))

Alternative Name: WNT10A

Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region.

Cellular Localization: Secreted , extracellular space , extracellular matrix

Molecular Weight: 46 kDa

Gene ID: 80326

Pathways: WNT Signaling