Pyruvate Kinase antibody

Catalog No. ABIN285893

The Goat Polyclonal anti-Pyruvate Kinase antibody has been validated for WB, ELISA and IHC. It is suitable to detect Pyruvate Kinase in samples from Rabbit.

Quick Overview for Pyruvate Kinase antibody (ABIN285893)

Target: Pyruvate Kinase (PK)

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This Pyruvate Kinase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Pyruvate Kinase Antibody

Cross-Reactivity (Details): Reacts with all forms of pyruvate kinase (pan M-PK). Cross reactivity agains Pyruvate Kinase from other mammalian tissues is expected but has not been specifically determined.

Purification: Ion exchange chromatography

Immunogen: Pyruvate kinase antibody (IgG fraction) was raised in goat using pyruvate kinase isolated from rabbit Muscle as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 1:4,000-1:20,000, IHC: 1:500-1:2,000, WB: 1:500-1:2,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details for Pyruvate Kinase

Target: Pyruvate Kinase (PK)

Alternative Name: Pyruvate Kinase

Background: Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP.