PLIN1 antibody (N-Term)

Catalog No. ABIN285897

This anti-PLIN1 antibody is a Guinea Pig Polyclonal antibody detecting PLIN1 in WB, IHC (p) and IHC (fro). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 22+ publications.

Quick Overview for PLIN1 antibody (N-Term) (ABIN285897)

Target: PLIN1 (Perilipin 1 (PLIN1))

Reactivity: Human, Mouse, Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This PLIN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PLIN1 Antibody

Binding Specificity: N-Term

Purification: Stabilized antiserum

Immunogen: Perilipin antibody was raised in guinea pig using duplicated N-terminus of perilipin as the immunogen.

Isotype: IgG1

Application Details

Application Notes: IHC-F: 1:100-200, IHC-P: 1:100-1:200, WB: 1:2,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as whole antiserum with 0.09 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C

References for anti-PLIN1 antibody (ABIN285897)

Bae, Hong, Lee, Jang, Lee, Choe, Offermanns, He, Lee, Koh: "Angiopoietin-2-integrin α5β1 signaling enhances vascular fatty acid transport and prevents ectopic lipid-induced insulin resistance." in: Nature communications, Vol. 11, Issue 1, pp. 2980, (2020) (PubMed).

Kong, Ji, Jeon, Han, Han, Lee, Lee, Jang, Choe, Baes, Kim: "Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5." in: Nature communications, Vol. 11, Issue 1, pp. 578, (2020) (PubMed).

Choi, Bae, Jeong, Park, Cho, Hong, Lee, Lee, Park, Suh, Choi, Yang, Jang, Onder, Moon, Jeong, Adams, Kim, Ludewig, Song, Lim, Koh: "YAP/TAZ direct commitment and maturation of lymph node fibroblastic reticular cells." in: Nature communications, Vol. 11, Issue 1, pp. 519, (2020) (PubMed).

Liu, Li, Li, Wang, Ding, Wang, Ye, Jin, Hou, Fang, Shu: "TREM2 regulates obesity-induced insulin resistance via adipose tissue remodeling in mice of high-fat feeding." in: Journal of translational medicine, Vol. 17, Issue 1, pp. 300, (2020) (PubMed).

An, Crewe, Asterholm, Sun, Chen, Zhang, Shao, Funcke, Zhang, Straub, Klein, Kusminski, Scherer: "Dysregulation of Amyloid Precursor Protein Impairs Adipose Tissue Mitochondrial Function and Promotes Obesity." in: Nature metabolism, Vol. 1, Issue 12, pp. 1243-1257, (2019) (PubMed).

Crewe, Joffin, Rutkowski, Kim, Zhang, Towler, Gordillo, Scherer: "An Endothelial-to-Adipocyte Extracellular Vesicle Axis Governed by Metabolic State." in: Cell, Vol. 175, Issue 3, pp. 695-708.e13, (2019) (PubMed).

Hepler, Shan, Zhang, Henry, Shao, Vishvanath, Ghaben, Mobley, Strand, Hon, Gupta: "Identification of functionally distinct fibro-inflammatory and adipogenic stromal subpopulations in visceral adipose tissue of adult mice." in: eLife, Vol. 7, (2019) (PubMed).

Monks, Orlicky, Stefanski, Libby, Bales, Rudolph, Johnson, Sherk, Jackman, Williamson, Carlson, MacLean, McManaman: "Maternal obesity during lactation may protect offspring from high fat diet-induced metabolic dysfunction." in: Nutrition & diabetes, Vol. 8, Issue 1, pp. 18, (2019) (PubMed).

Tran, Fitzgibbons, Min, DeSouza, Corvera: "Distinct adipocyte progenitor cells are associated with regional phenotypes of perivascular aortic fat in mice." in: Molecular metabolism, Vol. 9, pp. 199-206, (2019) (PubMed).

Xu, Li, Wu, Li, Zhao, Yu, Huang, Ferguson, Parton, Yang, Li: "Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions." in: The Journal of cell biology, Vol. 217, Issue 3, pp. 975-995, (2019) (PubMed).

Zhang, Hao, Shao, Nham, An, Wang, Zhu, Kusminski, Hassan, Gupta, Zhai, Sun, Scherer, Oz: "An Adipose Tissue Atlas: An Image-Guided Identification of Human-like BAT and Beige Depots in Rodents." in: Cell metabolism, Vol. 27, Issue 1, pp. 252-262.e3, (2019) (PubMed).

Shao, Vishvanath, Busbuso, Hepler, Shan, Sharma, Chen, Yu, An, Zhu, Holland, Gupta: "De novo adipocyte differentiation from Pdgfrβ+ preadipocytes protects against pathologic visceral adipose expansion in obesity." in: Nature communications, Vol. 9, Issue 1, pp. 890, (2018) (PubMed).

Fischer, Seki, Lim, Nakamura, Andersson, Yang, Honek, Wang, Gao, Chen, Samani, Zhang, Miyake, Oyadomari, Yasue, Li, Zhang, Liu, Cao: "A miR-327-FGF10-FGFR2-mediated autocrine signaling mechanism controls white fat browning." in: Nature communications, Vol. 8, Issue 1, pp. 2079, (2018) (PubMed).

Shin, Hwang, Choe, Park, Ji, Kim, Lee, Choi, Ching, Kovalik, Kim: "Macrophage VLDLR mediates obesity-induced insulin resistance with adipose tissue inflammation." in: Nature communications, Vol. 8, Issue 1, pp. 1087, (2018) (PubMed).

Hepler, Shao, Xia, Ghaben, Pearson, Vishvanath, Sharma, Morley, Holland, Gupta: "Directing visceral white adipocyte precursors to a thermogenic adipocyte fate improves insulin sensitivity in obese mice." in: eLife, Vol. 6, (2018) (PubMed).

Shao, Hepler, Vishvanath, MacPherson, Busbuso, Gupta: "Fetal development of subcutaneous white adipose tissue is dependent on Zfp423." in: Molecular metabolism, Vol. 6, Issue 1, pp. 111-124, (2018) (PubMed).

Lim, Hosaka, Nakamura, Cao: "Co-option of pre-existing vascular beds in adipose tissue controls tumor growth rates and angiogenesis." in: Oncotarget, Vol. 7, Issue 25, pp. 38282-38291, (2018) (PubMed).

Park, Kim, Sun, An, Gu, Scherer: "VEGF-A-Expressing Adipose Tissue Shows Rapid Beiging and Enhanced Survival After Transplantation and Confers IL-4-Independent Metabolic Improvements." in: Diabetes, Vol. 66, Issue 6, pp. 1479-1490, (2017) (PubMed).

An, Sun, Joffin, Zhang, Deng, Donzé, Kusminski, Scherer: "Angiopoietin-2 in white adipose tissue improves metabolic homeostasis through enhanced angiogenesis." in: eLife, Vol. 6, (2017) (PubMed).

Ye, Wang, Tao, Vishvanath, Shao, McDonald, Gupta, Scherer: "Impact of tamoxifen on adipocyte lineage tracing: Inducer of adipogenesis and prolonged nuclear translocation of Cre recombinase." in: Molecular metabolism, Vol. 4, Issue 11, pp. 771-8, (2015) (PubMed).

Target Details for PLIN1

Target: PLIN1 (Perilipin 1 (PLIN1))

Alternative Name: Perilipin

Background: Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, perilipin A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 and 46 kDa, respectively. The N-terminus, however, remains unchanged. The antiserum reacts specifically with perilipins (A and B) located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells.

Pathways: Lipid Metabolism