PP5 antibody (Atto 390)

Catalog No. ABIN2868701

This Mouse Monoclonal antibody specifically detects PP5 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for PP5 antibody (Atto 390) (ABIN2868701)

Target: PP5 (PPP5C) (Protein Phosphatase 5, Catalytic Subunit (PPP5C))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PP5 antibody is conjugated to Atto 390

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 12F7

Product Details anti-PP5 Antibody (Atto 390)

Specificity: Detects ~58 kDa.

Cross-Reactivity: Human

Purification: Protein G Purified

Immunogen: Full length human PP5 protein

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2868701 was sufficient for detection of PP5 in 15 μg of human A431 lysates by ECL analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for PP5

Target: PP5 (PPP5C) (Protein Phosphatase 5, Catalytic Subunit (PPP5C))

Alternative Name: PP5

Background: Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues, potentially playing a role in the regulation of cell growth and differentiation. PP5 or PPT is predominantly a nuclear protein and interacts with CDC16 and CDC27. It is found in association with several proteins that influence intracellular signlaing-cascades intitiated by hormones (glucocorticoids) or cellular stress (hypoxia, oxidative stress and DNA Damage). It phosphorylates serine residues of skeletal muscle phosphorylase and histone H1. It may also be involved in mitosis and RNA biogenesis regulation (1,2).

Gene ID: 5536

NCBI Accession: NP_006238

UniProt: P53041