The Rabbit Polyclonal anti-Acid Phosphatase antibody has been validated for WB and ELISA. It is suitable to detect Acid Phosphatase in samples from Potato.
ACP
Reactivity: Mouse
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 594
Application Notes
ELISA: 1:4000-1:20,000, WB: 1:500-1:2000 Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Concentration
Lot specific
Buffer
Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % gentamicin sulfate. DO NOT add NaN3.
Preservative
Without preservative
Handling Advice
Avoid repeated freeze/thaw cycles. Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Target
Acid Phosphatase (ACP)
Alternative Name
Acid Phosphatase
Background
Acid phosphatase is a phosphatase, a type of enzyme, used to free attached phosphate groups from other molecules during digestion. It is basically a phosphomonoesterase. It is stored in lysosomes and functions when these fuse with endosomes, which are acidified while they function, therefore, it has an acid pH optimum.