Ubiquitin antibody

Catalog No. ABIN2887820

The Rabbit Polyclonal anti-Ubiquitin antibody has been validated for WB. It is suitable to detect Ubiquitin in samples from Human, Mouse, Rat, Cow, Rabbit, Sheep and Guinea Pig.

Quick Overview for Ubiquitin antibody (ABIN2887820)

Target: Ubiquitin (Ub)

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Ubiquitin Antibody

Specificity: Detects a ~10kD protein, corresponding to the apparent molecular mass of ubiquitin on SDS-PAGE immunoblots, in samples from human, monkey, mouse, rat, hamster, rabbit, guinea pig, bovine, porcine, canine, sheep, chicken, Xenopus, yeast, Drosophila, E. coli and fish. It has also been shown to immunoprecipitate BSA-conjugated ubiquitin and will identify free ubiquitin and ubiquinated proteins on immunoblots.

Purification: Immunoaffinity purified

Immunogen: Ubiquitin purified from bovine red blood cells conjugated to KLH.

Type of Immunogen: Cells

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:10000)

Usage: Suitable for use in Western Blot. Western Blot: 1:10000 (ECL). Positive control: Mouse Brain Tissue Extract and Heat-shocked HeLa Cell Lysate.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway