TGF-beta antibody

Catalog No. ABIN289577

The Goat Polyclonal anti-TGF-beta antibody has been validated for WB, Neut, ELISA, FACS, IHC and ICC. It is suitable to detect TGF-beta in samples from Human.

Quick Overview for TGF-beta antibody (ABIN289577)

Target: TGF-beta (TGFb)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TGF-beta antibody is un-conjugated

Application: Western Blotting (WB), Neutralization (Neut), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-TGF-beta Antibody

No Cross-Reactivity: Human

Cross-Reactivity (Details): Does not react with human LAP derived TGF beta 2 precursor

Characteristics: Affinity purified Goat polyclonal TGF beta antibody
Bioactivity: ND50 is typically 15-30 µg/mL in the presence of 125 ng/mL ofrecombinant human LAP TGF beta 1 and 0.25 ng/mL TGF beta 1.

Purification: Purified

Sterility: Filtered

Endotoxin Level: <10 ng per 1 mg of the antibody as determined by the LAL method.

Immunogen: TGF beta antibody was raised in goat using CHO cell-derived recombinant human LAP (TGF-beta1) as the immunogen.

Isotype: IgG

Application Details

Application Notes: FC: 2.5 µg/10^6 cells, IHC: 5-15 µg/mL, ICC: 5-15 µg/mL, WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from a 0.2 µm filtered solution in PBS and NaCl with Trehalose.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for TGF-beta

Target: TGF-beta (TGFb)

Alternative Name: TGF beta

Background: Transforming growth factor (sometimes referred to as Tumor growth factor, or TGF) is used to describe two classes of polypeptide growth factors, TGF alpha and TGFBeta.

Pathways: EGFR Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus