FZD1 antibody (Cysteine-Rich Domain, Extracellular) (Biotin)

Catalog No. ABIN291497

The Goat Polyclonal anti-FZD1 antibody has been validated for WB and IHC. It is suitable to detect FZD1 in samples from Mouse.

Quick Overview for FZD1 antibody (Cysteine-Rich Domain, Extracellular) (Biotin) (ABIN291497)

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-FZD1 Antibody (Biotin)

Binding Specificity: Cysteine-Rich Domain, Extracellular

Specificity: Recognizes mouse Frizzled-1.

Purification: Immunoaffinity purified

Immunogen: NS0-derived recombinant, murine, Frizzled-1 extracellular Cysteine-rich Domain (CRD).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: Use at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~2 ng/lane under non-reducing and reducing. conditions. In this format, this antibody shows approximately 5 % cross-reactivity with rmFrizzled-3, rmFrizzled-4, rmFrizzled-7, rmFrizzled-8 and less than 1 % cross-reactivity with rmFrizzled-6. Immunohistochemistry: Detects Frizzled-1 in cells and tissues at 15 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile Tris-buffered saline  pH 7.3, containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.3, 0.1 % BSA.

Storage: -20 °C

Storage Comment: Lyophilized and resuspended forms: store at -20°C.

Target Details for FZD1

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Alternative Name: FZD1 / Frizzled 1

Background: Name/Gene ID: FZD1
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1

Gene ID: 8321

UniProt: Q9UP38

Pathways: WNT Signaling, Asymmetric Protein Localization