HSP70 antibody

Catalog No. ABIN291550

This Mouse Monoclonal antibody specifically detects HSP70 in WB, IHC, IF, IP, FACS, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Cow and Monkey.

Quick Overview for HSP70 antibody (ABIN291550)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human, Cow, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HSP70 Antibody

Specificity: Recognizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. Species cross-reactivity: Human, monkey and bovine.

Purification: Protein A purified

Immunogen: HSP70 protein from HeLa cells. Cellular Localization: Nuclear and cytoplasmic.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, IF, IHC, IHC-Fr, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native & denatured): 2 μg antibody/mg protein lysate using Protein A. Western Blot: 0.5-1 μg/mL for 2hrs at RT. Immunohistochemistry (Frozen & formalin/paraffin): 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: BT474 or HeLa cells, breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70 / Heat Shock Protein 70

Background: Name/Gene ID: HSP70

Synonyms: HSP70