ST14 antibody (AA 615-855)

Catalog No. ABIN292903

This Sheep Polyclonal antibody specifically detects ST14 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for ST14 antibody (AA 615-855) (ABIN292903)

Target: ST14 (Suppression of Tumorigenicity 14 (ST14))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This ST14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ST14 Antibody

Binding Specificity: AA 615-855

Specificity: Selected for its ability to recognize human Matriptase in direct ELISAs and Western Blots.

Purification: Affinity purified

Immunogen: E. coli-derived rhMatriptase (aa615-855).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Flow Cytometry, ELISA and Western Blot. Flow Cytometry: 25 μg/mL, 10 μL labels 1-5x10^5 cells in a total reaction volume 200 μL. Cells must first be fixed and permeabilized using 4 % paraformaldehyde, 0.1 % saponin in PBS. Tested using PC-3 cells. The binding of unlabeled antibodies may be visualized by adding an IgG (FITC). Direct ELISA: 0.5-1.0 μg/mL. Detection limit is ~0.5 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit is ~1 ng/lane under non-reducing and reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS with 5 % trehalose

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ST14

Target: ST14 (Suppression of Tumorigenicity 14 (ST14))

Alternative Name: ST14 / Matriptase

Background: Name/Gene ID: ST14
Subfamily: Serine S1
Family: Protease

Synonyms: ST14, Epithin, HAI, Prostamin, PRSS14, TADG15, Matriptase, MT-SP1, SNC19, TMPRSS14, MTSP1, Serine protease 14, Serine protease TADG-15, TADG-15

Gene ID: 6768

UniProt: Q9Y5Y6