c-FOS antibody

Catalog No. ABIN293678

The Sheep Polyclonal anti-c-FOS antibody has been validated for WB and ICC. It is suitable to detect c-FOS in samples from Human and Rat.

Quick Overview for c-FOS antibody (ABIN293678)

Target: c-FOS (c-Fos) (c-Fos)

Reactivity: Human, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This c-FOS antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-c-FOS Antibody

Specificity: Recognizes human c-fos. Species cross-reactivity: Rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide: Met-Phe-Ser-Gly-Phe-Asn-Ala-Asp-Tyr-Glu-Ala- Ser-Ser-Ser-Arg-Cys, selected from a conserved region of mouse and human c-fos.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, WB

Usage: Suitable for use in Western Blot and Immunocytochemistry. Western Blot: Detergent lysates were produced from HeLa and HL-60 cells stimulated to express c-fos protein. Detects a band at 55-60kD on immunoblots of stimulated HeLa and HL-60 lysates. Immunocytochemistry: Has been used in immunocytochemistry of vibratome sections of rat brains following either surgical damage to one of the hemispheres (detects c-fos and c-fos related proteins in the nuclei of neurons in the damaged hemisphere) or kainic acid treatment.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 10 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for c-FOS

Target: c-FOS (c-Fos) (c-Fos)

Alternative Name: FOS / c-FOS

Background: Name/Gene ID: FOS

Synonyms: FOS, Activator protein 1, AP-1, Cellular oncogene c-fos, Cellular oncogene fos, Proto-oncogene c-Fos, v-fos, C-FOS, G0s7, p55

Gene ID: 2353

UniProt: P01100

Pathways: S100 Proteins