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c-FOS antibody (AA 4-17)

This Sheep Polyclonal antibody specifically detects c-FOS in WB, IHC, IHC (p), IF and IHC (fro). It exhibits reactivity toward Human and Rat.
Catalog No. ABIN295405

Quick Overview for c-FOS antibody (AA 4-17) (ABIN295405)

Target

See all c-FOS (c-Fos) Antibodies
c-FOS (c-Fos) (c-Fos)

Reactivity

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Human, Rat

Host

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Sheep

Clonality

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Polyclonal

Conjugate

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This c-FOS antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Binding Specificity

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    AA 4-17

    Specificity

    Recognizes human c-fos. Species cross-reactivity: Rat.

    Purification

    Purified

    Immunogen

    Synthetic c-fos peptide (residues Ser Gly Phe Asn Ala Asp Tyr Glu Ala Ser Ser Ser Arg Cys) corresponding to amino acids 4-17 of human Fos, conjugated to bovine thyroglobulin.

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: IF, IHC, IHC-Fr (1:500 - 1:4000), IHC-P (1:500 - 1:4000), WB (10 μg/mL)

    Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 10 μg/mL Detects a band at ~39kD. Immunohistochemistry: 1:500-1:4000 (4?C, 1-3 days) Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase. Recommended fixation is 4 % PFA with 0.5 % picric acid (Li et al 1999). Triton X-100 pretreatment in the block only. Antigen retrieval for paraffin sections is recommended. Microwave citric acid treatment suggested.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    500 μL sterile ddH20

    Concentration

    Lot specific

    Buffer

    Lyophilized. No preservatives.

    Preservative

    Without preservative

    Handling Advice

    Avoid frequent freeze thaws. Store undiluted.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C.
  • Target

    c-FOS (c-Fos) (c-Fos)

    Alternative Name

    FOS / C-FOS

    Background

    Name/Gene ID: FOS

    Synonyms: FOS, Activator protein 1, AP-1, Cellular oncogene c-fos, Cellular oncogene fos, Proto-oncogene c-Fos, v-fos, C-FOS, G0s7, p55

    Gene ID

    2353

    Pathways

    S100 Proteins
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