c-FOS antibody (AA 4-17)
Quick Overview for c-FOS antibody (AA 4-17) (ABIN295405)
Target
See all c-FOS (c-Fos) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 4-17
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Specificity
- Recognizes human c-fos. Species cross-reactivity: Rat.
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Purification
- Purified
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Immunogen
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Synthetic c-fos peptide (residues Ser Gly Phe Asn Ala Asp Tyr Glu Ala Ser Ser Ser Arg Cys) corresponding to amino acids 4-17 of human Fos, conjugated to bovine thyroglobulin.
Type of Immunogen: Synthetic peptide -
Isotype
- IgG
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Application Notes
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Approved: IF, IHC, IHC-Fr (1:500 - 1:4000), IHC-P (1:500 - 1:4000), WB (10 μg/mL)
Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 10 μg/mL Detects a band at ~39kD. Immunohistochemistry: 1:500-1:4000 (4?C, 1-3 days) Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase. Recommended fixation is 4 % PFA with 0.5 % picric acid (Li et al 1999). Triton X-100 pretreatment in the block only. Antigen retrieval for paraffin sections is recommended. Microwave citric acid treatment suggested. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- 500 μL sterile ddH20
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Concentration
- Lot specific
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Buffer
- Lyophilized. No preservatives.
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Preservative
- Without preservative
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Handling Advice
- Avoid frequent freeze thaws. Store undiluted.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C.
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- c-FOS (c-Fos) (c-Fos)
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Alternative Name
- FOS / C-FOS
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Background
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Name/Gene ID: FOS
Synonyms: FOS, Activator protein 1, AP-1, Cellular oncogene c-fos, Cellular oncogene fos, Proto-oncogene c-Fos, v-fos, C-FOS, G0s7, p55 -
Gene ID
- 2353
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Pathways
- S100 Proteins
Target
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