FKBP4 antibody (full length, N-Term)

Catalog No. ABIN2962775

This anti-FKBP4 antibody is a Mouse Monoclonal antibody detecting FKBP4 in WB, IHC, IP, ICC and FACS. Suitable for Human, Mouse, Rat, Hamster and Dog.

Quick Overview for FKBP4 antibody (full length, N-Term) (ABIN2962775)

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Reactivity: Human, Mouse, Rat, Hamster, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This FKBP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: Hi52C

Product Details anti-FKBP4 Antibody

Binding Specificity: full length, N-Term

Specificity: Reacts with human FKBP52. Cross-reacts with mouse, rat, canine and hamster protein.

Purification: Purified (protein G)

Immunogen: Recombinant full-length FKBP52 with an N-terminal polyhistidine tag.

Isotype: IgG1

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
FC(1-2 μg for 10.6 cells) IHC(4 - 5 μg/mL) WB(0.5 - 1 μg/mL)

Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, glycerol 50 % .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium that is identical to that used in the assay system.

Storage: -20 °C

Storage Comment: Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FKBP4

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Alternative Name: FKBP52

Background: FK506-binding proteins, FKBP51 and FKBP52, are high molecular weight immunophilins that are part of the mature glucocorticoid receptor heterocomplex. The N-terminal domains of these proteins bind FK506 and have peptidyl-prolyl isomerase activity that converts prolyl peptide bonds within target proteins from cis- to transproline. The C-terminal domains contain the tetratricopeptide repeats that are involved in protein-protein interactions with Hsp90. Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptors differently and have different Hsp90-binding characteristics. In addition, FKBP51 is linked to the progesterone receptor while FKBP52 is linked to progesterone, androgen, and glucocorticoid receptors.

Peptidyl-prolyl cis-trans isomerase FKBP4, PPIase FKBP4 51 kDa FK506-binding protein, FKBP51 52 kDa FK506-binding protein, FKBP-52 59 kDa immunophilin, p59 FK506-binding protein 4, FKBP-4 FKBP59 HSP-binding immunophilin, HBI Immunophilin FKBP52 Rotamase Gene name : FKBP4

Gene ID: 2288

UniProt: Q02790

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway