GPR77 antibody (AA 1-50)

Catalog No. ABIN297118

This Rabbit Polyclonal antibody specifically detects GPR77 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Monkey and Cow.

Quick Overview for GPR77 antibody (AA 1-50) (ABIN297118)

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Reactivity: Human, Monkey, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR77 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPR77 Antibody

Binding Specificity: AA 1-50

Brand: IHC-plus™

Specificity: The amino acid sequence used as immunogen is 100 % homologous in human, and chimp (isoforms 1, 2, 3, and 4), 88 % homologous in rhesus monkey (isoforms 1, 2, 3), and 76 % homologous in bovine (isoforms 1 and 2).

Purification: Immunoaffinity purified

Immunogen: A portion of amino acids 1-50 of human GPR77.

Type of Immunogen: Protein fragment

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for GPR77

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Alternative Name: C5AR2 / GPR77 / C5L2

Background: Name/Gene ID: C5AR2
Subfamily: Anaphylatoxin
Family: GPCR

Synonyms: C5AR2, G-protein coupled receptor 77, GPF77, GPR77, C5L2, G protein-coupled receptor 77

Gene ID: 27202

UniProt: Q9P296