HAPLN4 antibody

Catalog No. ABIN297170

The Goat Polyclonal anti-HAPLN4 antibody has been validated for WB, ICC and IP. It is suitable to detect HAPLN4 in samples from Human.

Quick Overview for HAPLN4 antibody (ABIN297170)

Target: HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HAPLN4 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-HAPLN4 Antibody

Specificity: Selected for its ability to recognize human HAPLN4 in the applications listed. In Western Blots, this antibody shows less than 1 % cross-reactivity with rhHAPLN1.

Purification: Affinity purified

Immunogen: NS0-derived rhHAPLN4.

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IP, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human HAPLN4. The detection limit for rhHAPLN4 is approximately 10 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhHAPLN4 from conditioned media of transfected NS0 cells. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect HAPLN4 on SHSY5Y human neuroblastoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for HAPLN4

Target: HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4))

Alternative Name: HAPLN4

Background: Name/Gene ID: HAPLN4

Synonyms: HAPLN4, BRAL2, KIAA1926, Brain link protein 2

Gene ID: 404037

UniProt: Q86UW8