LILRA5 antibody (AA 17-248)

Catalog No. ABIN298859

The Goat Polyclonal anti-LILRA5 antibody has been validated for WB, ELISA, FACS and ICC. It is suitable to detect LILRA5 in samples from Rat.

Quick Overview for LILRA5 antibody (AA 17-248) (ABIN298859)

Target: LILRA5 (Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 5 (LILRA5))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This LILRA5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-LILRA5 Antibody

Binding Specificity: AA 17-248

Specificity: Selected for its ability to recognize rat LILRC1 in the applications listed.

Purification: Affinity purified

Immunogen: NS0-derived recombinant rat LILRC1 (aa17-248).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo, ICC (2 - 15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 1.5 ng/well. In this format, this antibody shows approximately 30 % cross-reactivity with rmLILRCI and less than 1 % cross-reactivity with rrLILRC2. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect rat LILRC1. The detection limit for rrLILRC1 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 2-15 μg/mL. This antibody will detect human rat LILRC1 in rat splenocytes. Flow Cytometry: Dilute to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome. This antibody has been tested on rat splenocytes for use in flow cytometry.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for LILRA5

Target: LILRA5 (Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 5 (LILRA5))

Alternative Name: Lilra5

Background: Name/Gene ID: LILRA5
Family: Immunoglobulin

Synonyms: LILRA5, CD85, CD85F, CD85f antigen, ILT-11, ILT11, LILRB7, Leukocyte Ig-like receptor 9, LIR-9, LIR9

Gene ID: 353514