CPE antibody (AA 42-453)

Catalog No. ABIN299519

The Goat Polyclonal anti-CPE antibody has been validated for WB, ELISA, IP and FACS. It is suitable to detect CPE in samples from Human.

Quick Overview for CPE antibody (AA 42-453) (ABIN299519)

Target: CPE (Carboxypeptidase E (CPE))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CPE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-CPE Antibody

Binding Specificity: AA 42-453

Specificity: Recognizes human Carboxypeptidase E. Cross-reactivity: Shows less than 1 % cross-reactivity with rhCPA1, rmCPA4 and rhCPB1 in ELISA and Western Blot.

Purification: Affinity purified

Immunogen: NS0-derived recombinant human Carboxypeptidase E (aa42-453).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 0.2 μg/mL), Flo, IP, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. ELISA: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~1 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit for rhCPE is ~5 ng/lane under reducing and non-reducing conditions. Flow Cytometry: (A172 cells): For intracellular staining cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μLto 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents. The detection limit is ~1 ng/well. Immunoprecipitation: Used to immunoprecipitate rhCPE from conditioned media of transfected NSO cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

Target Details for CPE

Target: CPE (Carboxypeptidase E (CPE))

Alternative Name: Carboxypeptidase e / CPE

Background: Name/Gene ID: CPE
Subfamily: Metallopeptidase M14
Family: Exopeptidase

Synonyms: CPE, Carboxypeptidase E, CPH, Carboxypeptidase H, Enkephalin convertase

Gene ID: 1363

UniProt: P16870

Pathways: Peptide Hormone Metabolism, Synaptic Membrane