MIF antibody (AA 2-32)

Catalog No. ABIN301756

Product Details anti-MIF Antibody

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Binding Specificity: AA 2-32

Reactivity: Human, Monkey

Host: Chicken

Clonality: Polyclonal

Conjugate: This MIF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Recognizes human mature Migration Inhibitory Factor, Macrophage (MIF) at 12.3kD. The MIF gene encodes a protein of 115aa. The initiating methionine is cleaved leaving a mature protein of 114aa.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Pig (94%) Sheep, Panda, Bovine, Horse, Rabbit (89%) Hamster (83%).

Purification: Purified

Immunogen: Eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). Peptide sequence: PMFIVNTNVPRASVPDGF- LSELTQQLAQATG. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Pig (94%), Sheep, Panda, Bovine, Horse, Rabbit (89%), Hamster (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgY

Application Details

Application Notes: Approved: ELISA (1:1000 - 1:2000), WB (1:500 - 1:1000)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:500-1:1000. Detects human MIF from supernatants or lysates of 2x1E6 endotoxin-stimulated PBMC stimulated for 24 hours with 1 % human serum plus 10 mg/mL E. coli LPS. ELISA: 1:1000-1:2000. Assayed against goat anti-chicken IgG (H&L) in a standard capture ELISA using ABTS as a substrate for 30 min at RT.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile dH2O

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF (MIF Products)

Background: Name/Gene ID: MIF

Synonyms: MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase

Gene ID: 4282

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling