HLA-DP/DR antibody (Biotin)

Catalog No. ABIN301998

Product Details anti-HLA-DP/DR Antibody (Biotin)

Target: HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DP/DR antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Anti-HLA-DR/DP Biotin

Specificity: The antibody MEM-136 recognizes a common extracellular epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: PHA-activated peripheral blood lymphocytes.

Clone: MEM-136

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-HLA-DP/DR antibody (Biotin) (ABIN301998)

Koch, Staffler, Hüttinger, Hilgert, Prager, Cerný, Steinlein, Majdic, Horejsí, Stockinger: "T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density." in: International immunology, Vol. 11, Issue 5, pp. 777-86, (1999) (PubMed).

Target Details for HLA-DP/DR

Target: HLA-DP/DR (MHC Class II HLA-DP/DR (HLA-DP/DR))

Alternative Name: HLA-DR/DP (HLA-DP/DR Products)

Background: HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.