LIM Domain Kinase 1 antibody (pThr508)

Catalog No. ABIN3020310

The Rabbit Polyclonal anti-LIM Domain Kinase 1 antibody has been validated for WB, IHC and IF. It is suitable to detect LIM Domain Kinase 1 in samples from Human.

Quick Overview for LIM Domain Kinase 1 antibody (pThr508) (ABIN3020310)

Target: LIM Domain Kinase 1 (LIMK1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-LIM Domain Kinase 1 Antibody

Binding Specificity: pThr508

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Phosphorylated Antibodies

Immunogen: A phospho specific peptide corresponding to residues surrounding T508 of human LIMK1

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:100,IF,1:100 - 1:200

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Alternative Name: LIMK1

Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.,LIMK1,LIMK,LIMK-1,Signal Transduction,Kinase,Serine/threonine kinases,Tyrosine kinases,Cell Biology & Developmental Biology,Cytoskeleton,Microfilaments,Microtubules,Actins,TGF-b-Smad Signaling Pathway,Immunology & Inflammation,Neuroscience,Cell Type Marker,Neuron marker,Growth Cone,Protein phosphorylation,LIMK1

Molecular Weight: 33 kDa/68 kDa/70 kDa/72 kDa

Gene ID: 3984

UniProt: P53667

Pathways: Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events